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. 2010 Jun 24;6(6):e1000958. doi: 10.1371/journal.ppat.1000958

Figure 3. Visualization of viral RNAs in VSV infected cells.

Figure 3

(A) Fluorescent microscopy images of BSR-T7 cells showing virus infection (red), viral RNA (green) and the cell nuclei (blue). Cells were infected (+Inf) with rVSV-RFP-P at an MOI of 3 or mock infected (−Inf). At 5 hpi, cells were depleted of UTP, treated with actinomycin D (+ActD) and where indicated transfected 1h later with 5mM BrUTP (+BrUTP). Following 1h incubation at 37°C to allow incorporation of BrUTP into RNA, cells were fixed and the RNA was detected using an Alexa Fluor-488 conjugated antibody against bromodeoxyuridine. The RFP-P protein was visualized at 561nm, and the cell nuclei were stained with DAPI. (B) Cells were infected and processed as in panel A, except that the duration of the BrUTP labeling was reduced to 30 minutes. Size bars = 5µm.