Figure 5. Viral RNA is transported away from inclusions in a microtubule-dependent manner.

(A) Schematic of the RNA pulse-chase experiment performed in (B). (B) BSR-T7 cells were infected with rVSV-RFP-P (MOI = 3) and RNA was labeled using the strategy outlined in A. Viral RNAs (green) and RFP-P (red) are shown following a 70-minute chase in which nocodazole was present (upper panels) or washed out (w/o, lower panels) for the last 30 min. (C) An image of an rVSV infected BSR-T7 cell showing some BrUTP labeled RNA (green) is associated with α-tubulin (red). Size bars = 5µm.