Figure 6. The VSV N, P and L proteins localize at the sites of RNA synthesis.

(A) Images of BSR-T7 cells infected with rVSV or rVSV-RFP-P showing the distribution of the viral replication machinery N, P and L (red) in relation to newly synthesized VSV RNA (green). Cells were infected at an MOI of 3 and at 4 hpi depleted of intracellular UTP and treated with ActD and nocodazole. Following a 1h incubation, cells were transfected with BrUTP and fixed 40 minutes later. N and L proteins and viral RNA were detected by antibody staining and images acquired by confocal microscopy. (B) Cells were treated as above. Triple labeling shows viral RNA (green) at the site of synthesis together with P (red) and L protein (blue). Size bars = 5µm.