Figure 1.

Analysis of mRNA levels in human atherosclerotic lesions by cDNA arrays. The mRNA was purified from the fibrous cap (lesion) or the tunica media (media) of 13 patients and analyzed in pooled groups by hybridization to cDNA arrays. (a) Storage phosphor image of 1 array containing 588 unique cDNAs double-spotted onto nitrocellulose and hybridized with ³²P-labeled, reverse-transcribed cDNA from human media acquired by endarterectomy of 6 pooled patients. (b) Quantitation from the storage phosphor screen was normalized to a mean housekeeping value (mean), which was the average of GAPDH, β-actin, and 23-kD HBP for each array. Thirteen patients were analyzed in groups on 3 array pairs (media versus lesion). Error bars, SEM; n = 3 experiments. HBP, highly basic protein.