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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Feb 20;1797(6-7):865–877. doi: 10.1016/j.bbabio.2010.02.016

Figure 4. Rates of ROS production and O2 consumption under FET and RET.

Figure 4

Freshly isolated mitochondria from guinea pig heart were resuspended and analyzed as described in Methods and the legend of Fig. 3. A) Under FET (G/M) and RET (Succ), H2O2 and several other bioenergetic variables (e.g. ΔΨm, NAD(P)H) were monitored simultaneously with a spectrofluorometer (see legend Fig. 3 and Methods). B) In parallel, and under similar conditions, O2 measurements were performed. Mitochondrial respiration was measured in state 3 (St3) and state 4 (St4). Shown are the results obtained with H2O2 (A) and O2 (B) specific fluxes. The numbers in grey represent the H2O2 detected as a percentage of the O2 consumed (see Methods). The number of samples, n, analyzed was n=4 (2 experiments) and n=6 (2 experiments) for panels A and B, respectively. * p<0.05; ** p<0.01; *** p<0.001.