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. 2010 Jun 24;6(6):e1000965. doi: 10.1371/journal.ppat.1000965

Figure 6. Inhibition of deacetylase activity fails to rescue the replication of pUL29/28 or pUL38-deficient viruses.

Figure 6

(A) Trichostatin A does not rescue pUL29/28 or pUL38-deficient viruses. Fibroblasts were infected at a multiplicity of 0.1 pfu/cell using wild-type (BADwt) or inoculum containing equivalent numbers of viral genomes for BADsubUL29 (subUL29) or BADsubUL38 (subUL38) viruses. Cultures were treated with 300 nM TSA for the duration of the experiment and viral titers from culture supernatants were determined at 10 dpi. Data is from duplicate experiments. (B) BADsubUL29 (subUL29) virus is deficient in immediate-early gene expression irrespective of treatment with TSA. Cells pretreated with 500 nM of TSA were infected using 0.1 pfu/cell of wild-type (BADwt) or BADsubUL29 (subUL29) virus as described above, and total RNA was harvested at the indicated times. Expression of IE1 RNA was determined by qRT-PCR using cDNA produced from DNase-treated RNA and random hexamers. Quantitation was completed with SYBR green and primers specific to exon 4 of IE1. Samples were normalized to cellular GAPDH RNA levels.