Table 4.
Nrf2-regulated mouse hepatic proteins determined in iTRAQ analysis 2 (test set).
| SwissProt acc. no. | Name | Relative expression compared to Nrf2(+/+) mouse 1 |
Fold change Nrf2(−/−) /Nrf2(+/+) | p | |||||
|---|---|---|---|---|---|---|---|---|---|
| Nrf2(+/+) |
Nrf2(−/−) |
||||||||
| Geometric mean | Lower 95% CI | Upper 95% CI | Geometric mean | Lower 95% CI | Upper 95% CI | ||||
| P10649 | Glutathione S-transferase Mu 1 | 1.00 | 0.91 | 1.10 | 0.44 | 0.40 | 0.47 | 0.44 | 0.001 |
| P17717 | UDP-glucuronosyltransferase 2B5 | 0.99 | 0.93 | 1.06 | 0.55 | 0.52 | 0.57 | 0.55 | 0.001 |
| Q8VCC2 | Liver carboxylesterase 1 | 1.06 | 0.96 | 1.17 | 0.59 | 0.54 | 0.64 | 0.56 | 0.001 |
| Q91X77 | Cytochrome P450 2C50 | 0.97 | 0.87 | 1.08 | 0.56 | 0.46 | 0.69 | 0.58 | 0.001 |
| P19157 | Glutathione S-transferase P 1 | 0.95 | 0.89 | 1.01 | 0.58 | 0.54 | 0.63 | 0.62 | 0.001 |
| Q9D379 | Epoxide hydrolase 1 | 0.97 | 0.90 | 1.05 | 0.63 | 0.59 | 0.68 | 0.65 | 0.001 |
| Q64458 | Cytochrome P450 2C29 | 1.08 | 0.90 | 1.29 | 0.75 | 0.62 | 0.90 | 0.69 | 0.001 |
| P30115 | Glutathione S-transferase A3 | 1.03 | 0.98 | 1.08 | 0.72 | 0.66 | 0.78 | 0.70 | 0.001 |
| P24549 | Retinal dehydrogenase 1 | 0.94 | 0.86 | 1.04 | 0.68 | 0.58 | 0.80 | 0.72 | 0.021 |
| O70475 | UDP-glucose 6-dehydrogenase | 1.09 | 0.97 | 1.23 | 0.79 | 0.63 | 0.99 | 0.73 | 0.183 |
| Q62452 | UDP-glucuronosyltransferase 1-9 | 0.99 | 0.93 | 1.06 | 0.73 | 0.58 | 0.92 | 0.74 | 0.183 |
| Q91VA0 | Acyl-coenzyme A synthetase ACSM1, mitochondrial | 0.97 | 0.91 | 1.04 | 0.79 | 0.74 | 0.84 | 0.81 | 0.001 |
| Q64442 | Sorbitol dehydrogenase | 1.02 | 0.92 | 1.13 | 0.84 | 0.78 | 0.91 | 0.83 | 0.081 |
| P97494 | Glutamate-cysteine ligase catalytic subunit | 1.15 | 1.06 | 1.25 | 0.95 | 0.89 | 1.02 | 0.83 | 0.021 |
| Q8CG76 | Aflatoxin B1 aldehyde reductase member 2 | 1.06 | 1.01 | 1.11 | 0.88 | 0.81 | 0.96 | 0.83 | 0.013 |
| Q9CQX2 | Cytochrome b5 type B | 1.01 | 0.91 | 1.13 | 0.85 | 0.76 | 0.94 | 0.83 | 0.197 |
| Q9JII6 | Alcohol dehydrogenase [NADP+] | 1.01 | 0.98 | 1.04 | 0.86 | 0.80 | 0.92 | 0.85 | 0.003 |
| Q8VCW8 | Acyl-CoA synthetase family member 2, mitochondrial | 1.00 | 0.93 | 1.08 | 0.86 | 0.79 | 0.93 | 0.86 | 0.132 |
| O55022 | Membrane-associated progesterone receptor component 1 | 1.03 | 0.96 | 1.11 | 0.89 | 0.81 | 0.98 | 0.86 | 0.207 |
| P47738 | Aldehyde dehydrogenase, mitochondrial | 1.02 | 0.99 | 1.06 | 0.88 | 0.85 | 0.92 | 0.86 | 0.000 |
| Q8QZS1 | 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial | 1.07 | 1.00 | 1.13 | 0.94 | 0.89 | 1.00 | 0.89 | 0.084 |
| Q9ET01 | Glycogen phosphorylase, liver form | 0.98 | 0.96 | 1.00 | 0.87 | 0.80 | 0.94 | 0.89 | 0.081 |
| O35945 | Aldehyde dehydrogenase, cytosolic 1 | 0.97 | 0.93 | 1.01 | 0.86 | 0.83 | 0.89 | 0.89 | 0.024 |
| Q8VDJ3 | Vigilin | 1.10 | 1.05 | 1.15 | 0.99 | 0.94 | 1.04 | 0.90 | 0.069 |
| Q9EQ20 | Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial | 1.00 | 0.98 | 1.03 | 0.92 | 0.87 | 0.98 | 0.92 | 0.140 |
| Q9Z2I8 | Succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial | 1.02 | 0.99 | 1.06 | 0.96 | 0.92 | 0.99 | 0.93 | 0.121 |
| Q99P30 | Peroxisomal coenzyme A diphosphatase NUDT7 | 0.98 | 0.93 | 1.03 | 1.10 | 1.05 | 1.17 | 1.13 | 0.039 |
| Q9CW42 | MOSC domain-containing protein 1, mitochondrial | 0.95 | 0.90 | 1.00 | 1.09 | 1.04 | 1.15 | 1.15 | 0.095 |
| Q9QXD6 | Fructose-1,6-bisphosphatase 1 | 1.02 | 0.96 | 1.09 | 1.21 | 1.10 | 1.33 | 1.18 | 0.117 |
| P31786 | Acyl-CoA-binding protein | 1.01 | 0.94 | 1.07 | 1.19 | 1.06 | 1.34 | 1.18 | 0.207 |
| P24369 | Peptidyl-prolyl cis-trans isomerase B | 0.95 | 0.89 | 1.01 | 1.12 | 1.05 | 1.20 | 1.18 | 0.017 |
| Q8VDM4 | 26 S proteasome non-ATPase regulatory subunit 2 | 0.90 | 0.80 | 1.02 | 1.08 | 1.00 | 1.17 | 1.20 | 0.183 |
| P06151 | L-lactate dehydrogenase A chain | 0.97 | 0.89 | 1.05 | 1.16 | 1.06 | 1.28 | 1.20 | 0.086 |
| Q61207 | Sulfated glycoprotein 1 | 0.94 | 0.84 | 1.05 | 1.14 | 1.07 | 1.21 | 1.21 | 0.057 |
| P16460 | Argininosuccinate synthase | 1.02 | 0.93 | 1.13 | 1.27 | 1.16 | 1.40 | 1.25 | 0.038 |
| Q3THE2 | Myosin regulatory light chain MRLC2 | 1.13 | 1.03 | 1.24 | 1.46 | 1.25 | 1.70 | 1.29 | 0.117 |
| Q8VBT2 | L-serine dehydratase | 1.02 | 0.91 | 1.15 | 1.37 | 1.13 | 1.67 | 1.34 | 0.183 |
| Q05816 | Fatty acid-binding protein, epidermal | 1.17 | 0.96 | 1.43 | 2.10 | 1.69 | 2.60 | 1.79 | 0.005 |
All values are expressed relative to a wild type control mouse (WT1). Proteins listed were significantly different in the null mice compared with wild type controls (Benjamini–Hochberg; p ≤ 0.2).