Table 6.
Proteins regulated by Nrf2 identified by 2DE analysis.
| Protein spot | SwissProt acc. No. | Protein | Mr/pI | Normalized spot Intensity (% total spot intensity) |
Fold change |
p | |
|---|---|---|---|---|---|---|---|
| Nrf2(+/+) | Nrf2(−/−) | (Nrf2(−/−)/ Nrf2(+/+)) | |||||
| 1 | P24549 | Aldehyde dehydrogenase family 1, subfamily A1 | 54468/7.91 | 0.19 ± 0.01 | 0.15 ± 0.01 | 0.78 | 0.0025 |
| 2 | P10649 | Glutathione S-transferase, mu 1 | 25970/7.7 | 0.88 ± 0.20 | 0.53 ± 0.07 | 0.60 | 0.0158 |
| 3 | P30115 | Glutathione S-transferase, alpha 3 | 25361/8.76 | 0.85 ± 0.09 | 0.67 ± 0.02 | 0.80 | 0.0098 |
| 4a | P19157 | Glutathione S-transferase, pi 1 | 23609/7.69 | 2.06 ± 0.17 | 1.20 ± .50 | 0.58 | 0.0170 |
| 4b | P19157 | Glutathione S-transferase, pi 1 | 23609/7.69 | 0.29 ± 0.03 | 0.017 ± 0.05 | 0.57 | 0.0040 |
| 4c | P19157 | Glutathione S-transferase, pi 1 | 23609/7.69 | 0.21 ± 0.01 | 0.11 ± 0.05 | 0.50 | 0.0073 |
| 5 | Q923D2 | Biliverdin reductase B | 22197/6.49 | 0.13 ± 0.01 | 0.010 ± 0.01 | 0.83 | 0.0155 |
| 6 | P11588 | Major urinary protein 6 | 20648/5.0 | 0.46 ± 0.06 | 0.17 ± 0.05 | 0.36 | 0.0002 |
Proteins from the livers of individual mice were separated by 2-DE as described in the Materials and methods. The protein spots were quantified from colloidal Coomassie blue-stained gels using ImageMasterTM 2D Elite software. Spot intensities were normalized to the total spot intensity for each gel and expressed as the mean percentage value ± SD (n = 4 for each group). Proteins that were significantly different (Student's t-test; p < 0.05) between the wild type and Nrf2 null mice are shown.