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. Author manuscript; available in PMC: 2010 Dec 21.
Published in final edited form as: Bioconjug Chem. 2009 Jul 31;20(8):1474–1481. doi: 10.1021/bc800421f

Figure 3.

Figure 3

(A) Flow cytometry analysis of cys-diabody conjugated Qdot binding with different tumor cells. Cells were treated with no protein (solid grey), mock conjugated Qdot 655 (dotted black line) and anti-HER2 iQdot 655 (solid black line). FL3 (λem: 670 nm long pass) was the filter used for Qdot 655. (B) Competitive cell binding assay by flow cytometry. An anti-HER2 antibody fragment, minibody (29) was used as competitor. Samples were assayed in triplicate and means ± SEM are shown, normalized to the signal obtained in the absence of competitor.