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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: J Thromb Haemost. 2010 Feb 17;8(5):1107–1115. doi: 10.1111/j.1538-7836.2010.03812.x

Figure 1.

Figure 1

Figure 1

Effect of thrombin on the expression of Ang/Tie2 signaling molecules. EA.hy926 cells were incubated with indicated concentrations of thrombin for 12h either before (panels A, C, and E) or after (panels B, D, and F) treating monolayers with PC-S195A (50 nM, 30 min). Protein levels in cell lysates for Tie2 (A and B), Ang1 (C and D) and Ang2 (E and F) were measured by ELISA. * p< 0.05 as compared to 0 nM Th. Time course of the thrombin-mediated expression of Ang1 (G) and Ang2 (H) in the PC-S195A-pretreated cells were measured after incubation with 2 nM thrombin followed by measuring protein levels in cell lysates by ELISA. Symbols are: ○, PC-S195A; ●, thrombin + PC-S195A; □, thrombin alone. *p< 0.05 as compared to 0 nM Th.