Figure 4.
Effects of different proteases on the expression of Tie2, Ang1, and Ang2 proteins. Primary HUVECs were incubated with indicated proteases (2 nM thrombin, 20 nM APC or GD-APC) for 16h with (black bars) or without (white bars) sequential treatment with MβCD (10 mM, 1h) and PC-S195A (50 nM, 30 min) followed by measuring expression levels of Tie2 (A) Ang1 (B) and Ang2 (C) by ELISA. *p< 0.05 as compared to without MβCD.