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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: J Thromb Haemost. 2010 Feb 17;8(5):1107–1115. doi: 10.1111/j.1538-7836.2010.03812.x

Figure 5.

Figure 5

S1P1- and PI3K-dependent thrombin regulation of the expression of Tie2, Ang1, and Ang2. The PC-S195A pretreated primary HUVECs were incubated with thrombin (2 nM, 12h) before or after transfection with control or specific siRNA for S1P1 (10 nM, 24h) or treatment with LY294002 (10 μM, 1h). Expression levels of Tie2 (A) Ang1 (B) and Ang2 (C) were measured by ELISA. .*p< 0.05 as compared to 2 nM Th + PC-S195A. (D) Primary HUVECs were preincubated with blocking antibodies to EPCR, PAR-1, or Tie2 (25 μg/mL, 30min) or transfected with siRNA for Tie2 (10 nM, 24h) followed by incubation with PC-S195A and stimulation by thrombin (2 nM for 12h). The cell permeability was measured as described under “Materials and Methods”. *p< 0.05 as compared to PC-S195A alone. NS, non-specific