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Recurrence of the same peptide in different MS2-only and MS2 + MS3 scans in the same sample. Shown is the precursor ion m/z and retention time (RT) of one identified peptide AEEDEILNRpSPR in 3 different MS2-only scans (A, B, C) and 3 different MS2 + MS3 scans (D, E, F) in the NM-2C5 cell sample enriched with ZrO2. The very similar retention time strongly supports the utility of integrated quantitative method. The arrow indicates the spectrum where the peptide was identified.
