Figure 1.
Overexpression of Keap1 in HepG2 cells drives down Nrf2 levels and is accompanied by increased colony forming ability, as well as morphological plasticity. A) Immunoblot of cells stably expressing FLAG/Luciferase or FLAG/Keap1; B) Colony forming ability; C) Cell proliferation. Cells expressing FLAG/Luciferase exhibited a doubling time of approximately 31 hrs. Cells expressing FLAG/Keap1 exhibited a doubling time of approximately 24 hrs; D) Light microscopic images (200 × magnification).