Figure 6.
Loss of E-Cadherin is accompanied by increased expression of Slug. A) E-Cadherin immunofluorescence in control and Nrf2 shRNA containing A549 cells expressed from retrovirus; B) Immunoblotting for E-Cadherin in control and Nrf2 shRNA containing A549 cells expressed from retrovirus; C) Immunoblot of Slug expression in control and Nrf2 shRNA containing A549 cells expressed from retrovirus; D) Wild type A549 cells were transiently co-transfected with a luciferase reporter under the control of the human E-Cadherin promoter, a plasmid vector that expressed β-galactosidase, a vector that expressed human wild type Nrf2, or insertless pcDNA3.1. Luciferase/β-Gal activity was measured 72 hrs after transfection.