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. 2010 Jun 24;5(6):e11305. doi: 10.1371/journal.pone.0011305

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Gawlik et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Left panels, the biosensor and PA83 were cleaved by furin (1 h; 37°C) at the indicated enzyme-substrate ratio. Right panel, the biosensor was cleaved by WNV NS2B-NS3 proteinase (1 h; 37°C) at the indicated enzyme-substrate ratio. The cleavage reactions were analyzed by SDS-gel electrophoresis followed by Coomassie staining.