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. 2010 Jun 24;5(6):e11305. doi: 10.1371/journal.pone.0011305

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Gawlik et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Left panel, the time course of the biosensor cleavage by U251, MCF-7:furWT and MCF-7:fur:D153N cells (5×10⁴). Right panel, SDS-gel electrophoresis of the cleavage reactions. Cells (5×10⁴) were incubated for 4 h with the biosensor (100 pmol). After centrifugation, the supernatant samples were separated by SDS-gel electrophoresis followed by Coomassie staining. Purified furin (10 fmol and 100 fmol) was used as a control.