Figure 12. The biosensor cleavage in cell lysates.

Left panel, Triton X-100 (0.1%) does not affect the efficiency of furin proteolysis of the biosensor. The biosensor was co-incubated with purified furin (5 fmol and 50 fmol) for 0-120 min with or without 0.1% Triton X-100. Right panel, the biosensor was co-incubated 1 h with purified furin at the indicated enzyme-substrate molar ratio. The digests were analyzed by SDS-gel electrophoresis followed by Coomassie staining. Where indicated, reactions contained 0.1% Triton X-100.