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. Author manuscript; available in PMC: 2010 Jun 25.
Published in final edited form as: Anal Chem. 2010 Jun 1;82(11):4621–4627. doi: 10.1021/ac1007915

Figure 4.

Figure 4

Automated cell counting accuracy of the lens-free imaging technique is illustrated at various RBC densities, ranging from <50 000 cells/μL up to ~0.4 × 106 cells/μL. For each dilution level, at least 500 cells were automatically counted to estimate the cell density in each case. The reconstructed cell images yielded an absolute error rate of <5%, up to a cell density of ~0.4 × 106 cells/μL. The inset also illustrates a comparison of RBC volume histogram that is estimated based on holographic reconstructions against a commercially available hematology analyzer (Model Coulter LH750, Beckman Coulter), which showed a very good fit to our results.