Table 1.
Cell type | Mitochondria | Plastids | Nuclei | Cytosol | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Control | Cadmium | % change | Control | Cadmium | % change | Control | Cadmium | % change | Control | Cadmium | % change | |
Mesophyll | 375 (15.0)a | 249 (16.0)b | −34*** | 37 (2.0)a | 21 (2.0)b | −43*** | 43 (6.0)a | 20 (2.0)c | −30* | 39 (2.0)a | 24 (4.0)b | −38** |
Long-stalked trichome | 351 (26.0)a | 262 (23.0)b | −25* | 43 (4.0)a | 28 (4.0)b | −33* | 59 (5.0)b | 14 (2.0)c | −76*** | 34 (2.0)a | 9 (2.0)c | −74*** |
Short-stalked trichome | 351 (13.0)a | 256 (14.0)b | −27*** | 40 (4.0)a | 22 (2.0)b | −45** | 50 (2.0)ab | 15 (1.0)c | −70*** | 33 (1.0)a | 8 (0.5)c | −76*** |
Values are means with standard errors (parenthesis) and document the amount of gold particles bound to glutathione per µm2 found in different cell structures (mesophyll and glandular trichomes) of control and cadmium treated Cucurbita pepo (L.) leaves. Significant differences in the amounts of gold particles between cell structures of mesophyll cells, short- and long-stalked glandular trichome cells are indicated by different lowercase, superior letters. Samples which are significantly different from each other have no letter in common. P < 0.05 was regarded significant analyzed with Kruskal–Wallis test followed by post-hoc comparison according to Conover. Significant differences were also calculated for the % changes in the amounts of gold particles with the Mann–Whitney U test between cadmium treated and control cells. n > 20 for plastids and 60 for all other cell structures
*P < 0.05, **P < 0.01, and ***P < 0.001