Table 2.
Antibody | Specificity | Labeling |
---|---|---|
JIM 5 | Middle to low methyl esterified pectins (up to 40% esterified)a | CW in general, stronger staining at growth zones in cell center and tips, mucilage vesicles. Enhanced labeling after de-esterification |
JIM7 | Middle to high methyl esterified pectins (15% to 80% esterified)a | Growing zones of CW at cell center and tips. Labeling diminishes after de-esterification |
2F4 | De-esterified pectinsa | Labeling only after de-esterification at whole CW |
Anti-mucilage | Mucilage | Newly formed tips after cell division, non-growing zones before division, dictyosomes, secretory vesicles |
aFor references, see “Material and methods”
CW cell wall