Fig. (2).

(A) Ventricular tissue taken from mice is subjected to trypsin digestion. Peptides are then separated on the basis of charge and hydrophobicity. (B) Eluted peptides are then electrosprayed into a Linear Ion-Trap Mass Spectrometer via electrospray ionization (ESI). The resultant mass spectra are matched to proteins using database algorithms. (C) Increasing salt concentrations, or “salt bumps”, elute peptides from the LC columns providing enhanced peptide separation. Used with Permission: Chugh et al., Large-scale studies to identify biomarkers for heart disease: a role for proteomics? Expert Opin Med Diagn 2009; 3(2): 133-141.