Fig. 2.

Change in protein levels related to mitochondrial fission or fusion in diabetic MCECs. Western blots showing OPA1, MFN1 and MFN2 (mitochondrial fusion-related proteins), and FIS1 and DRP1 (mitochondrial fission-related proteins). Actin was used as a loading control. OPA1 is significantly decreased in MCECs isolated from diabetic mice compared with MCECs from control mice (control, 1.00 ± 0.10: diabetic, 0.52 ± 0.03; p < 0.05, n = 4 samples for each group), whereas DRP1 is significantly increased in MCECs from diabetic mice (control, 1.03 ± 0.29; diabetic, 2.40 ± 0.21; p < 0.05, n = 4 samples for each group)