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. Author manuscript; available in PMC: 2011 Jul 9.
Published in final edited form as: J Mol Biol. 2010 May 7;400(2):204–217. doi: 10.1016/j.jmb.2010.05.003

Figure 2.

Figure 2

Determination of tRNA parameters of Trm5. (A) Single turnover kinetics of the m1G37 synthesis reaction at 55°C performed at the indicated concentrations of M. jannaschii Trm5 with tRNA (0.25 mM) and AdoMet (25 µM). (B) Plot of the rate vs. concentration data fit to a hyperbolic equation to derive kchem and Kd for tRNA. (C) Pre-steady-state kinetics of the m1G37 synthesis reaction by M. jannaschii Trm5 (1 µM)-m1G37-tRNA (0–1.5 µM) complexes with tRNA (10 µM) and AdoMet (50µM). (D) Plot of the rate vs. concentration data fit to a quadratic equation to deriveKd for m1G37-tRNA.