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. Author manuscript; available in PMC: 2011 Jul 9.
Published in final edited form as: J Mol Biol. 2010 May 7;400(2):204–217. doi: 10.1016/j.jmb.2010.05.003

Figure 3.

Figure 3

Determination of AdoMet parameters of Trm5. (A) Pre-steady-state kinetics of the m1G37 synthesis reaction by rapidly mixing M. jannaschii Trm5 (5 µM)-AdoMet (0.4–9.6 µM) complexes with tRNA (15 µM). (B) Plot of the rate vs. concentration data of (A) fit to a hyperbolic equation to derive the Kd for tRNA. (C) Pre-steady-state kinetics of the m1G37 synthesis reaction by rapidly mixing M. jannaschii Trm5 (5 µM)-AdoHcy (0–0.2 µM) complexes with tRNA (15 µM) and AdoMet (25 µM). (D) Plot of the data of (C) fit to a hyperbolic equation to derive Kd for AdoHcy.