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. 2010 May 11;61(11):2979–2990. doi: 10.1093/jxb/erq125

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© 2010 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 5. — The function of NO in H₂O₂-regulated GA biosynthesis. (a) Effects of H₂O₂, NO, and their production inhibitor DPI or scavenger c-PTIO, or the GA biosynthesis inhibitor PAC on the dormancy and germination of freshly harvested seed; the germination ratio was counted on the seventh day. (b) Changes in the transcript levels of GA20ox GA biosynthesis genes under different treatments at 24 h imbibition. (c) Changes in the transcript levels of GA3ox GA biosynthesis genes under different treatments at 24 h imbibition. (d) Changes in the transcript levels of GA-regulated genes XTH5 and EXP2 under different treatments at 24 h imbibition. H₂O₂ (10 mM), DPI (10 μM), SNP (200 μM), c-PTIO (200 μM), and PAC (10 μM) were used for these experiments. Values are the means±SE (n=3 for a–d). An ANOVA test followed by a rank test was performed. Different letters are used to indicate means that are significantly different (P <0.05).