Cells were treated with the indicated concentrations of (a) IL-17A, (b) IL-22, or (c) TNF-α for 24 hours. Cells were treated with the optimal cytokine concentrations of (d) IL-17A, (e) IL-22, or (f) TNF-α for 6, 24, or 48 hours. Cell-free supernatants were harvested and CCL20 protein levels were quantified by ELISA for all experiments. All experiments were performed in triplicate at least three separate times and the average protein levels and SD are reported. Significant differences were detected using the Mann–Whitney unpaired two-tailed t-test (*Indicates significance, P<0.05).