Table 1.
Relative levels of leptin-induced phosphorylated signaling intermediaries in endometrial epithelial cells (EEC)
Cell type |
pSTAT3 |
pERK 1/2 |
pAKT1 |
---|---|---|---|
Primary EEC | 2.4a | 1.9a | 1.3a |
HES | 1.4a | 2.5a | 1.6a |
Ishikawa | 1.5a | 2.2a | 1.5a |
SK-UT2 | 1.4a | 1.3a | 1.4a |
An3Ca | n.s. | 1.3a | 1.7a |
Note: The data represent quantitative analysis of phosphorylated molecules from benign and cancer EEC after incubation for 20 min with leptin (62.5 nM). Data are derived from densitometry analysis of bands observed on film calculated by the NIH Image program, (http://rsb.info.nih.gov/nih-image) or the Li-COR Odyssey Software. The values were normalized to the non-phosphorylated corresponding protein and expressed on a fold difference from basal expression in vehicle treated cells (control; n ≥ 3). (n.s.) no significant difference with respect to control
P <0.05 significant difference with respect to control.