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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Clin Exp Pharmacol Physiol. 2010 Apr;37(4):e136–e144. doi: 10.1111/j.1440-1681.2010.05343.x

Figure 1.

Figure 1

The unique morphology and replication pattern of Dot cells. Dot cells were sorted using E-cadherin antibody from adult mouse blood and then cultured in αMEM with 20% FBS. Dot cells have an unusual self-renewal pattern. The newly replicated Dot cells group as circular shape (arrows), and are released from the colony (1A). These grouped Dot cells migrate away from the colony and spread in a scattered pattern throughout the plate (1B). When Dot cells reach confluence, large spheroids forms on top of the monolayer (1C). This spheroid constantly releases grouped Dot cells (arrows) that migrate away. A large spheroid is located on top of the Dot cell monolayer with a shell-like structure at the outer layer of this spheroid (arrow in 1D). Using 100× oil lens plus 1.5× zoom, 1E shows a merged confocal image of a cultured spheroid. Bars in 1A, 1C and 1D = 40 µm; bar in 1B = 160 µm; bar in 1E = 10 µm.