Figure 4.
In vitro expanded Dot cells accelerate wound healing in diabetic mice. A 1.5 cm2 open wound was created on the dorsal skin of each diabetic mouse before 1.5 million cultured Dot cells were transplanted through tail-vein injection. The control group received saline. Three mice were used for each group. 12-day (4A) and 24 day (4B) healed wounds on Dot cell-transplanted and control diabetic mice. Newly grown hairs (arrows) surrounded the scar of each Dot cell-transplanted mouse. 4C shows quantitative wound area during the healing. The surfaces of wound areas were measured daily or every another day and the data was calculated. Accelerated healing was observed in Dot cell-transplanted diabetic mice by the tenth day after wounding. *P value = ≤ 0.05 was considered significant.