Figure 8.
Differentiated cells are released from Dot cell spheroids in vivo. Figure 8A shows that both hair follicles and dermal cells express GFP. The GFP was expressed in whole follicle cells in the undifferentiated hair follicles (thick arrows), but only expressed in the cells at the inner sheath area of the differentiated hair follicles (thin arrows). A merged image shows a GFP spheroid with DAPI stained nuclei embedded inside (arrows in 8B). Figure 8C shows the confocal images of a 5-day wound section after GFP-labeled Dot cell-transplantation. Bar in 8A = 50 µm. Image in 8B was taken by 100 × oil lens and 2× zoom. Bar in 8C = 10 µm.