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. Author manuscript; available in PMC: 2011 Feb 16.
Published in final edited form as: Biochemistry. 2010 Feb 16;49(6):1281–1289. doi: 10.1021/bi902001a

FIGURE 2.

FIGURE 2

FIGURE 2

FIGURE 2

FIGURE 2

Product inhibition studies to determine the substrate binding order. Assays were performed by varying the concentration of one substrate at a fixed concentration of the second substrate and in the presence of one of the products of the reaction. (A) NADH varied in the presence of NAD+ (0 (□), 55 (○) and 110 µM (Δ)) with DD-CoA fixed at 35 µM. (B) DD-CoA varied in the presence of NAD+ (0 (○), 55 (Δ) and 110 µM (□)) with NADH fixed at 250 µM. (C) NADH varied in the presence of lauryl-CoA (0 (○), 50 (□) and 100 µM (Δ)) with DD-CoA fixed at 35 µM. (D) DD-CoA varied in the presence of lauryl-CoA (0 (○), 50 (□) and 100 µM (Δ)) with NADH fixed at 250 µM.