Table 1.
Primers Used for Cloning and Mutagenesis
| Name | Sequence a |
|---|---|
| bmFabV forward | 5’GGAATTCCATATGATCATCAAACCGCGCGTACGC3’ |
| bmFabV reversed | 5’CGCGGATCCTCATTCGATCAGATTCGGAATCCG3’ |
| Y235A forward | 5’GTCACGCACGACATCGCCTGGAACGGCTCGAT3’ |
| Y235A reversed | 5’ATCGAGCCGTTCCAGGCGATGTCGTGCGTGAC3’ |
| Y235S forward | 5’ACGCACGACATCTCCTGGAACGGCT3’ |
| Y235S reversed | 5’AGCCGTTCCAGGAGATGTCGTGCGT3’ |
| K244A forward | 5’ATCGGCGAAGCGGCGAAAGATCTCGACC3’ |
| K244A reversed | 5’GGTCGAGATCTTTCGCCGCTTCGCCGAT3’ |
| K244R forward | 5’GATCGGCGAAGCGAGGAAAGATCTCGACC3’ |
| K244R reversed | 5’GGTCGAGATCTTTCCTCGCTTCGCCGATC3’ |
| K245M forward | 5’CGAAGCGAAGATGGATCTCGACCGC3’ |
| K245M reversed | 5’GCGGTCGAGATCCATCTTCGCTTCG3’ |
| K244A/K245A forward | 5’ATCGGCGAAGCGGCGGCAGATCTCGACCG3’ |
| K244A/K245A reversed | 5’CGGTCGAGATCTGCCGCCGCTTCGCCGAT3’ |
| ftuACP forward | 5’GGAATTCCATATGAGTACACATAACGAAGATTCTAAA3’ |
| ftuACP reversed | 5’CCGCTCGAGACCTACATCTTTAGATTCGATATA-3’ |
a
Restriction sites and mutated sites are shown in underline.