. Author manuscript; available in PMC: 2011 Feb 16.

Published in final edited form as: Biochemistry. 2010 Feb 16;49(6):1281–1289. doi: 10.1021/bi902001a

Table 2.

Kinetic Parameters for Wild-type and Mutant bmFabV Enzymes

Enzyme	k_cat (min⁻¹)	K_m (µM)		k_cat/K_m (µM⁻¹min⁻¹)

		DD-CoA	NADH	DD-CoA
wild-type without His-tag	1728 ± 10	4.4 ± 0.3	ND	392 ± 27
wild-type with His- tag	1242 ± 30	2.5 ± 0.4	23 ± 3	497 ± 79
Y235A	5.0 ± 0.1	2.7 ± 0.2	23 ± 2	1.8 ± 0.1
Y235S	6.1 ± 0.3	2.2 ± 0.1	6 ± 1	2.8 ± 0.1
K244A	11 ± 1	7.2 ± 0.6	66 ± 2	1.5 ± 0.1
K244R	1.3 ± 0.1	7.6 ± 0.7	69 ± 1	0.17 ± 0.01
K245M	18 ± 1	28 ± 2	28 ± 2	0.64 ± 0.03
K244A/K245A	ND	ND	65 ± 4	0.023 ± 0.002