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. Author manuscript; available in PMC: 2010 Jun 25.
Published in final edited form as: J Biol Chem. 2007 Nov 28;283(4):1962–1973. doi: 10.1074/jbc.M706877200

FIGURE 10. The importance of the SLN1-SKN7 pathway increases under wall stress.

FIGURE 10

Strains JF2007 (sln1Δ) and JF2368 (sln1Δccw12Δ) carrying the PTP2 expression plasmid to allow viability were transformed with plasmids pCLM994 (SLN1+) or pCLM1994 (sln1D1144N) and grown to log phase in YPD or YPD + 1 m sorbitol, and serial dilutions were spotted onto YPD, YPD + hygromycin B (25 μg/ml), and YPD + Congo Red (30 μg/ml) plates to which sorbitol (1 m) was added where indicated (bottom). Plates were photographed after 48 h of incubation at 30 °C.