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. Author manuscript; available in PMC: 2010 Jun 25.
Published in final edited form as: J Biol Chem. 2007 Nov 28;283(4):1962–1973. doi: 10.1074/jbc.M706877200

FIGURE 5. Hygromycin B-sensitive wall mutants do not activate the SLN1-SKN7 pathway.

FIGURE 5

Strains were Kan::MX marked deletions from the haploid Research Genetics Deletion Collection assembled based on their known functions in wall integrity and tested for hygromycin B sensitivity and activation of the SLN1-SKN7 pathway-dependent POCH1-lacZ reporter gene. A, hygromycin B sensitivity test. Strains were grown to log phase in YPD and 10-fold dilutions spotted on YPD or YPD containing 25 μg/ml hygromycin B plates, which were incubated at 30 °C for 48 h and then scanned. B, β-galactosidase was measured in a at least three transformants carrying the POCH1-lacZ reporter, pZL1320 (diagonal striped bars), or the POCH1(min)-lacZ reporter, pSL1156 (dark bars). Activity is represented in Miller units and normalized to protein concentration. The number below the plot is the ratio of average activity for the mutant (POCH1 reporter/POCH1(min) control reporter) over the average activity for a wild type (WT) strain measured in the same experiment.