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Northern (RNA) hybridization analysis of RNA was prepared from wild type (wt) (JF1565) and skn7D427N (JF2336) cultures after 60 min of treatment with 1 unit/ml zymolyase (+) or without treatment (−). OCH1 and NCA3 expression was normalized to expression of the SLN1-SKN7-independent CDC33 gene.
