TABLE 1.
Strains used in this study
| Genotype | Derivation | |
|---|---|---|
| JF1565 | MATα canR cyhR his3Δ200 leu2Δ1 ura3-52 trp1Δ63 lys2Δ202 | Ref. 1 |
| JF1732 | MATα fps1Δ::LEU2 canR cyhR his3Δ200 leu2Δ1 ura3-52 trp1Δ63 lys2Δ202 | fps1Δ::LEU2 derivative of JF1565 (1) |
| JF1904 | MATα skn7Δ::TRP1 canR cyhR his3Δ200 leu2Δ1 ura3-52 trp1Δ63 lys2Δ202 | skn7Δ::TRP1 derivative of JF1565 (10) |
| JF1919 | MATΔ ssk1::LEU2 canR cyhR his3Δ200 leu2Δ1 ura3-52 trp1Δ63 lys2Δ202 | Ref. 45 |
| JF2007 | MATα sln1ΔLEU2 his3Δ200 leu2Δ1 ura3-52 trp1Δ63 pRS426-PTP2 | Also known as RJY1428 (21) |
| JF2008 | MATα sln1ΔLEU2 sho1ΔLEU2 his3Δ200 leu2Δ1 ura3-52 trp1Δ63 pRS426-PTP2 | sho1Δ::LEU2 derivative of JF2007; one-step replacement |
| JF2154 | MATα, ssk1Δ::LEU, ypd1Δ::KanMX, sln1Δ::TRP1, his3Δ200, leu2Δ1, ura3-52, trp1Δ63, lys2Δ202 | |
| JF2162 | MATa canR cyhR, skn7Δ::TRP1, ssk1Δ::LEU2, his3Δ200, leu2Δ1, ura3-52, trp1Δ63, lys2Δ202 | |
| JF2181 |
MATa ssk1Δ::LEU2 sln1Δ::TRP1 canR cyhR his3Δ200 leu2Δ1 ura3-52 trp1Δ63 lys2Δ202 |
Product of a cross between JF2154 and JF2161 |
| JF2278 | MATα ccw12Δ::KanMX canR cyhR his3Δ200 leu2Δ1 ura3-52 trp1Δ63 lys2Δ202 | ccw12Δ::KanMX derivative of JF1565; one-step replacement |
| JF2279 | MATα, skn7Δ::TRP1, ccw12Δ::KanMX canR cyhR his3Δ200 leu2Δ1 ura3-52 lys2Δ202 | ccw12Δ::KanMX derivative of JF1904; one-step replacement |
| JF2300 | MATα ssk1Δ::LEU2 sln1Δ::TRP1 ccw12Δ::KanMX canR cyhR his3Δ200 ura3-52 lys2Δ202 | ccw12Δ:KanMX derivative of JF2181; one-step replacement |
| JF2301 | MATα ssk1Δ::LEU2 ccw12Δ::KanMX canR cyhR his3Δ200 ura3-52 lys2Δ202 trp1Δ63 | ccw12Δ:KAN derivative of JF1919; one-step replacement |
| JF2336 | MATα skn7D427N canR cyhR his3Δ200 leu2Δ1 ura3-52 trp1Δ63 lys2Δ202 | Derivative of JF1904; one-step replacement using fragment from pSL237 |
| JF2337 | MATα sed1Δ::HIS3 canR cyhR his3Δ200 leu2Δ1 ura3-52 trp1Δ63 lys2Δ202 | sed1Δ::HIS3 derivative of JF1565; one-step replacement |
| JF2338 | MATα sed1Δ::HIS3 ccw12Δ::KanMX canR cyhR his3Δ200 leu2Δ1 ura3-52 trp1Δ63 lys2Δ202 | ccw12Δ:KanMX derivative of JF2337; one-step replacement |
| JF2368 | MATa sln1ΔLEU2 his3Δ200 leu2Δ1 ura3-52 trp163 ccw12Δ::KanMX pRS426-PTP2 | ccw12Δ::KAN derivative of JF2007; one-step replacement |
| JF2437 | MATa ppz1Δ::KAN canR cyhR his3Δ200 leu2Δ1 ura3-52 trp1Δ63 | ppz1Δ::KAN derivative of JF1562; one-step replacement |
| JF2438 | MATα ppz2Δ::KAN canR cyhR his3Δ200 leu2Δ1 ura3-52 trp1Δ63 lys2Δ201 | ppz2Δ::KAN derivative of JF1565; one-step replacement |
| JF2441 | MATα ppz1Δ::KanMX ppz2Δ::KanMX his3Δ200 leu2Δ1 ura3-52 trp1Δ63 | Product of cross between JF2437 and JF2438 |