TABLE 3.
SKN7 and SLN1 dependence of ccw12 activation of the OCH1 SLN1-SKN7 pathway target gene
| Genotype |
β-Galactosidase activity (S.D.),a (POCH1-lacZb) |
Induction (ccw12Δ/CCW12) |
|---|---|---|
| SKN7 CCW12c | 320.8 (25.8) | 1.0 |
| SKN7 ccw12Δ | 538.9 (72.4) | 1.7 |
| skn7D427N ccw12Δ | 123.5 (30.1) | 0.4 |
| skn7Δ ccw12Δ | 26.5 (10.2) | 0.08 |
| SLN1 SSK1 CCW12 d | 360.8 (71.2) | 1.0 |
| SLN1 SSK1 ccw12Δ | 743.7 (59.9) | 2.1 |
| SLN1 ssk1Δ CCW12 | 136.6 (30.1) | 1.0 |
| SLN1 ssk1Δ ccw12Δ | 474.9 (44.3) | 3.5 |
| sln1Δ ssk1Δ CCW12 | 40.3 (13.9) | 1.0 |
| sln1Δ ssk1Δ ccw12Δ | 19.1 (0.8) | 0.5 |
a
β-Galactosidase activities are given in Miller units normalized to protein concentration and represent the averages of three measurements. Standard deviations are given in parentheses.
b
Each strain is transformed with the CEN based POCH1-lacZ, pZL1320 (10).
c
CCW12 skn7Δ, JF1904; ccw12 skn7Δ (JF2279) strains carry the wild type (pSL232) or D427N (pSL237) alleles of SKN7 on CEN plasmids, and strains lacking SKN7 carry the empty pRS315 vector.
d
Strains include CCW12 SSK1 SLN1, JF1565; CCW12 ssk1Δ SLN, JF1919; ccw12Δ SSK1 SLN1, JF2278; ccw12Δ ssk1Δ SLN1, JF2301; CCW12 ssk1Δ sln1Δ, JF2181; ccw12Δ ssk1Δ sln1Δ, JF2300.