TABLE 4.
Effect of ccw12 on intracellular glycerol
| Genotypea | Glycerolb (S.D.) |
Relative glycerol |
β-Galactosidase activityc (S.D.) POCH1-lacZd |
Relative POCH1-lacZ |
|---|---|---|---|---|
| Wild type | 6.3 (1.6) | 1.0 | 113.1 (19.5) | 1.0 |
| ccw12Δ | 7.5 (1.4) | 1.2 | 312.6 (14.7) | 2.8 |
| fps1Δ | 23.9 (3.2) | 4.1 | 298.5 (45.8) | 2.6 |
a
Wild type, JF1565; ccw12Δ, JF2278 and fps1ΔA, JF1732 are shown.
b
Glycerol (mg/g) values are the average of six determinations per strain.
c
β-Galactosidase values are given in Miller units normalized to protein concentration and are the average of three measurements per strain.
d
Each strain is transformed with the CEN based POCH1-lacZ reporter, pZL1320 (10).