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. Author manuscript; available in PMC: 2011 Jun 30.

Published in final edited form as: J Am Chem Soc. 2010 Jun 30;132(25):8787–8794. doi: 10.1021/ja102849w

Measurements of forward commitment factors of PfOPRT and HsOPRT. [1'-³H]Orotidine was pre-incubated with OPRTs. The mixture was diluted with a large excess of cold orotidine and various concentrations of PPi. The fractions of formed product from bound substrate were plotted versus the corresponding PPi concentrations. The forward commitment factors (C_f) were calculated from the equation $C_{f} = \frac{Y_{max}}{1 - Y_{max}}$ , where Y_max is the y axial intercept upon extrapolating the plateau region. The values of C_f are 0.043 ± 0.002 for PfOPRT () and 0.031 ± 0.004 for HsOPRT ().

Inline graphic — Measurements of forward commitment factors of PfOPRT and HsOPRT. [1'-³H]Orotidine was pre-incubated with OPRTs. The mixture was diluted with a large excess of cold orotidine and various concentrations of PPi. The fractions of formed product from bound substrate were plotted versus the corresponding PPi concentrations. The forward commitment factors (C_f) were calculated from the equation $C_{f} = \frac{Y_{max}}{1 - Y_{max}}$ , where Y_max is the y axial intercept upon extrapolating the plateau region. The values of C_f are 0.043 ± 0.002 for PfOPRT () and 0.031 ± 0.004 for HsOPRT ().