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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Clin Immunol. 2010 Mar 25;136(1):130–138. doi: 10.1016/j.clim.2010.02.014

Figure 5. Concentrated pDC suspensions produce downstream type 1 interferon-inducible transcripts and proteins, augmented by addition of IFNα.

Figure 5

(A) Interferon-inducible transcripts produced by TLR9 stimulated >92% pure pDC suspensions measured by real-time quantitative PCR. Amplification of pDC production of type 1 interferon-inducible transcripts in the presence of small amounts of added IFNα-2a protein (25 pg/ml). 10,000 pDCs in 50 µl volumes used. (B) The interferon-inducible protein Mx1 is present in intracellular fractions and the ubiquitin-like modifier and cytokine protein ISG15 is secreted into cell media at 24 hours. (C) Further downstream effects of type 1 interferon signaling in which multiple intracellular proteins have been conjugated by ISG15 is evident. Unstimulated pDCs (No Tx) do not have or secrete detectable ISG15 or ISG15 conjugates. IFNAR blockade prevents ISG15 conjugation. Transcripts measured by quantitative real-time PCR.