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Effect of Aktl AO on Aktl mRNA expression in various human cancer cells. The cells were transfected without (−) or with 0.3 µM of Akt1 AO (+) using Lipofectamine Plus for 3 h and incubated for 6 h with fresh media containing 10% FBS. Total RNA was isolated and Akt1 mRNA expression was determined using RT-PCR. β-Actin was determined as a loading control. Representative data are shown from two independent cell culture experiments and each RT-PCR reaction was performed in duplicate.
