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Oxidative refolding of reduced RNase. Reduced RNase 10 μM (80 μM –SH) in 50 mM Tris buffer, pH 7.5, 25 °C was incubated with 5 μM reduced PDI and 50 nM QSOX (squares); 50 nM QSOX alone (triangles); 5 μM reduced PDI and 1 mM GSH and 0.2 mM GSSG (circles); or 1 mM GSH and 0.2 mM GSSG alone (diamonds). At the times indicated samples were quenched with NEM and assayed for RNase using cCMP (see Experimental Procedures).
