Table 1.
Incresed extract ability: comparisons between conventional versus vigorous extraction, and each values of the Sephadex G50
| Sample | Amount of 1,3-β-glucan (μg mg−1)a | Amount of total phenolics (μg mg−1)b | Value of absorbance, relative (fold increase) | |
|---|---|---|---|---|
| 280 nm | 370 nm | |||
| Extract from conventional hot-water extraction | 0.2 | 35.1 | 35.6 (1) | 7.1 (1) |
| Extract from vigorous extraction (unpurified) | 316.5 | 65.2 | 202.0 (×5.7) | 43.5 (×6.1) |
| Sephadex G-50 fractions | ||||
| F-I | 317.3 | 6.3 | 0.3 | 0.1 |
| F-II | 0.02 | 41.8 | 1.1 | 0.6 |
| F-III | 0.001 | 17.1 | 0.9 | 0.4 |
aDetermined by using the modified Limulus test, which is specific for β–glucan (3). See total sugar content by phenol–sulfuric a acid method in Figure 1; bDetermined using Folin–Ciocalteu method (4).