Skip to main content
. 2010 May 14;29(12):2082–2096. doi: 10.1038/emboj.2010.81

Figure 1.

Figure 1

Knockdown of ATF4 inhibits tumour cell survival and proliferation. (A) ATF4 protein levels from the nuclear fractions of thapsigargin-treated (Tg, 1 μM, 4 h) or DMSO-treated cells. Lamin A/C was used as a loading control. (B) Survival of HT1080 and DLD1 cells cultured in the presence or absence of NEAA (100 μM) measured by MTT assay 48 h after plating (Data represent mean±s.e.m., n=3, *P<0.05). Cell survival was normalized to control (shNT cells without NEAA). (C) Cell proliferation assay. HT1080 cells were plated in DMEM with/without NEAA for 24 h. (Left panel) Fluorescent staining for nuclei (Hoechst, blue) and proliferating cells with EdU (red). (Right panel) Three randomly selected photographs were selected and numbers of EdU positive and total nuclei were counted. Percentage proliferation index was calculated by dividing the number of proliferating nuclei by the total number of nuclei. (Data represent mean±s.e.m., n=3, *P<0.05).