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Knockdown of ATF4 increases sensitivity to glutamine deprivation. (A) The biosynthetic reaction catalysed by ASNS. (B) Survival of HT1080 (left panel) or DLD1 (right panel) cells grown in DMEM with/without 4 mM L-glutamine for 48 h. (C) Survival of HT1080 cells in DMEM with/without Gln or Asn for 48 h. Gln: 4 mM, Asn: 100 μM. (Data represent mean±s.e.m., n=3, *P<0.05).
