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. 2010 May 14;29(12):2014–2025. doi: 10.1038/emboj.2010.85

Figure 2.

Figure 2

p18Hamlet accumulates at the myogenin promoter at early stages of muscle differentiation. (A) Schematic representation of the mouse myogenin gene indicating the TATA box and the transcription start site. The positions of the DNA fragments amplified with three different pairs of primers are indicated. ‘a' refers to a non-coding region located 18 kb upstream, ‘b' to the TATA box-containing region, and ‘c' to a region close to the TATA box of the myogenin promoter. (B) ChIP analysis of p18Hamlet, Histone H3 and H2A.Z binding to the genomic regions ‘a', ‘b' and ‘c' of the myogenin promoter. qPCR data are shown as means±s.d. of samples from a representative experiment assayed in triplicates. In each case, relative binding to the ‘b' and ‘c' regions is referred to the binding to the ‘a' region, which is given the value of 1. (C) p18Hamlet binding to the myogenin promoter (region ‘b') was assayed by ChIP in proliferating C2C12 myoblasts (GM) and after 14 h in DM, either in the presence or absence of SB203580 (SB), as indicated. Both semiquantitative (left panel) and quantitative (right panel) PCRs were performed.