Skip to main content
. 2010 May 14;29(12):2014–2025. doi: 10.1038/emboj.2010.85

Figure 8.

Figure 8

Mammalian SRCAP is required for muscle differentiation. (A) C2C12 myoblasts were transfected with siRNAs to downregulate YL-1 or H2A.Z expression. Cyclophylin B siRNA was used as a control. The efficiency of siRNA-induced target mRNA downregulation was analysed 48 h after transfection by qRT–PCR. (B) C2C12 myoblasts were transfected with the indicated siRNAs and 48 h after transfection, cells were placed in DM. The formation of muscle fibres was analysed 4 days later, both by phase contrast microscopy (upper panel, bar=250 μm) and by immunofluorescence with MHC antibodies (lower panel, bar=30 μm). (C) C2C12 myoblasts were transfected with H2A.Z or YL-1 siRNAs and either cultured in GM or incubated in DM for the indicated times. Total cell lysates were analysed by immunoblotting with the indicated antibodies. (D) C2C12 myoblasts were cultured in GM and transfected with either control or YL-1 siRNAs. Chromatin was obtained 72 h after transfection, and H2A.Z binding to the myogenin-containing TATA region was assayed by ChIP. Both quantitative and semiquantitative PCR results are shown. (E) C2C12 myoblasts were transfected with siRNAs to downregulate the expression of SRCAP and p400 ATPases and cultured in GM or incubated in DM for the indicated times. Myogenin expression was analysed by immunoblotting using tubulin as a loading control. (F) C2C12 myoblasts were transfected with SRCAP or p400 siRNAs and the expression of the target mRNAs was analysed 48 h after transfection by qRT–PCR.