Figure 3.

RanBP1 induces movement of HEAT9 loop by recruiting the C-terminal tail of Ran. Close-up view into the region where the C-terminal tail of Ran clashes with HEAT9 loop is shown. (A) Yeast CRM1:RanBP1:RanGTP complex. (B) CRM1:Spn1:RanGTP complex (CRM1 is mouse, whereas Spn1 and Ran are human) (Monecke et al, 2009). Colouring is the same as in Figure 2. Dashed lines in light blue indicate hydrogen bonds or salt bridges. GTP is shown as space-filling spheres. Residue numbering differs between species. Comparison of the two structures suggests that the C-terminus of Ran (blue in (A)) would clash with HEAT9 loop (magenta) and displace it on RanBP1 binding.